95 / 220
Table 12. Spike and recovery data for AND High Protein Placebo. Samples were spiked with
FloraGlo 5% lutein at the initial sample dilution step.
Native
level
(μg/100
g)
Spike
(μg/100
g)
Recovery RSD
Spike
(μg/100
g)
Recovery RSD
Spike
(μg/100
g)
Recovery RSD
Lutein
3.3
486.4
101.1% 0.5%
708.8
101.2% 0.5%
1425.3
100.7%
0.2%
Table 13. Spike and recovery data for Child Formula Placebo. Samples were spiked with
standards at the internal standard step of sample preparation.
Native
level
(μg/100
g)
Spike
(μg/100
g)
Recovery
RSD
Spike
(μg/100
g)
Recovery
RSD
Lutein
0.1
5.5
101.8%
0.2%
16.8
100.8%
0.3%
β-Carotene
0.6
5.3
107.2%
0.2%
16.2
104.2%
0.3%
Table 14. Spike and recovery data for Elemental Formula Placebo. Samples were spiked with
standards at the internal standard step of sample preparation.
Native
level
(μg/100
g)
Spike
(μg/100
g)
Recovery
RSD
Spike
(μg/100
g)
Recovery
RSD
Lutein
ND
5.5
103.7%
0.2%
16.8
101.9%
0.6%
β-Carotene
0.1
5.3
104.1%
0.2%
16.2
103.5%
0.6%
ND Not detected
Table 15. Spike and recovery data for PN Infant Formula RTF. Samples were spiked with
standards at the internal standard step of sample preparation.
Native
level
(μg/100
g)
Spike
(μg/100
g)
Recovery
RSD
Spike
(μg/100
g)
Recovery
RSD
Lutein
3.9
6.2
98.7%
0.4%
18.4
98.6%
0.1%
β-Carotene
16.4
5.9
100.6%
0.9%
17.7
100.1%
0.6%
Acknowledgments
Shang-Jing Pan and Douglas Gordon of Abbott Nutrition for reviewing the method and
providing feedback.
Steve Tennyson and Scott Christiansen of Perrigo Nutritionals for reviewing the method and
validation data.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE