85 / 220
Figure 3. Linearity plot for β-carotene.
LOD/LOQ
The limit of detection (LOD) and limit of quantitation (LOQ) were extrapolated from the
signal-noise ratio calculated in ChemStation software when measuring analyte concentrations
of 1.4-1.7 mcg/100 g. LOD was calculated as (3 * measured conc)/signal-noise ratio. LOQ
was calculated as (10 * measured conc)/signal-noise ratio. Results from three different spiked
samples were averaged. The LOQ for both lutein and β-carotene meet that of ≤1 μg/100 g
specified in the SMPR.
Table 4. LOD and LOQ for lutein in spiked SRM 1849a.
Sample
Measured conc
(mcg/100 g as
fed)
S/N
Extrapolated LOD
(mcg/100 g)
Extrapolated LOQ
(mcg/100 g)
1
1.40
68.6
0.06
0.20
2
1.46
70.4
0.06
0.21
3
1.43
37.0
0.12
0.39
Avg
0.08
0.27
Table 5. LOD and LOQ for β-carotene in spiked SRM 1849a.
Sample
Measured conc
(mcg/100 g as fed)
S/N
Extrapolated
LOD (mcg/100
g)
Extrapolated
LOQ
(mcg/100 g)
1
1.62
61.6
0.08
0.26
2
1.70
61.2
0.08
0.28
3
1.66
61.0
0.08
0.27
Avg
0.08
0.27
VALIDATION REPORT
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