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Technical Assessment: Operational Suitability
Time-consuming multi-step
process
• May take hours to days
• Time dependent on the sample,
protocols, selected platform and analysis
complexity
Protocol Complexity
• Multiple step process (e.g., DNA/RNA
extraction, nucleic acids fragmented and
end-repaired, sequencing, analysis)
Operational suitability for any
approach will be assessed with
BioWatch laboratories and other
SMEs before being considered
for operational use
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FOR OFFICIAL USE ONLY
Technical Assessment: Laboratory Suitability
How would we optimize protocols for BioWatch
?
• What type of sample prep is needed?
• How can time requirements be minimized?
– Example: How much time does it take per run?
• How much sample is needed?
• What is the depth and quality?
• What are the sequencing biases and errors?
Can we trust the analytical results
?
• When do I call something present?
– One read? Many? Stacked reads or broad coverage?
– Contamination and host cross-interaction?
– Determine abundance from read counts?
• How do I evaluate the probability that the result is accurate?
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