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7
Technical Assessment: Sequencing Working
Group
Discussion and analysis based on August 2016 National
Academy of Sciences discussion regarding the potential for use
of amplicon sequencing
Focused on technical assessment of metagenomic and amplicon
sequencing
• Document and/or leverage existing sequencing activities across
multiple agencies and laboratories
• Assess strengths and weaknesses of approaches
Membership — Sequencing Experts
• Federal agencies
• BioWatch laboratories
• Other laboratory experts
Meetings or teleconferences on a monthly or bi-weekly
basis
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Informing the Sequencing Working Group
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Development of Program Requirements
• “Value to stakeholder” sequencing discussion with local laboratory
directors/designees
• Identify level of characterization required to inform public health decisions
• Develop stakeholder questionnaire
• Effectiveness criteria
• Suitability criteria
• Feedback generated will inform the Program requirement development and
discussions of the Sequencing Working Group
Identify opportunities to leverage clinical
and non-clinical applications; areas of
overlap as well as separation
Evaluate targeted amplicon sequencing as
well as metagenomics