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3/9/2017
7
22
27
19
14
11
11
0
10
20
30
Instrument
GC/LC column Column specification
GC column
temperature
LC gradient program SPE cartridge
The comprehensive instrument, analyticalparameters and SPE cartridge of AOAC collaborative study
Technicalconditions
GC-MS (16 Labs)
GC-MS/MS(15 Labs)
LC-MS/MS (24 Labs)
Instrument
（
22
）
5 types: Agilent; Varian
5 types: Agilent; Waters; Thermo Fisher and
Bruker
12 types: Agilent; SHIMADZU; Waters and
Thermo Fisher
Chromatographic column
（
27
）
5 brands
：
DB-1701;Restek Rtx 1701; MR2; HP-5
ms; HP-5MSUI
8 brands
：
DB-1701;HP-5 ms; HP-5MSUI; HP-5MSI;
DB-5MSUI; DB5-MS; BR-5ms; Rxi 5 Sil
14 brands
：
ZORBAXSB-C18;phenomenex Luna C8; Waters
Atlantis C18; phenomenex Luna C18
Specification
（
19
）
30m×0.25mm×0.25μm
3 Specification
：
30m×0.25mm×0.25μm; 15m×0.25mm×0.25μm;
length20m/Diam. 0.180mm/Film 0.18µm
15 Specification
：
3.5 μm×100mm ×2.1mm;
3.5μm×150mm×2.1;150mm×2.00mm×3μm
①
GCcolumn
temperature (14)
②
LCgradient
program(11)
5 GC column temperature
：
40℃ hold 1 min , at 30℃/min to 130℃,
at 5℃/min to 250℃, at 10℃/min to 300
℃, hold 5 min
9 GC column temperature
：
40℃ hold 1 min , at 30℃/min to 130℃, at
5℃/min to 250℃, at 10℃/min to 300 ℃, hold
5 min
11 GC column temperature :
0min-99%A, 3 min-70% A, 6 min-60% A, 9 min-
60%A, 15 min -40% A, 19 min -1% A, 23 min -1%
A, 23.01 min -99% A
SPE cartridge(12)
3 SPE cartridges
：
CleanertTPT; UCT (ECPSACB506);
Envicarb/PSA
4 SPE cartridges
：
CleanertTPT; InertSep GC/PSA; Agilent
Technolongies BE
Carbon 500mg/PSA; Envi Carb/PSA
5 SPE cartridges
：
CleanertTPT; InertSep GC/PSA; Agilent
Technolongies BE Carbon 500mg/PSA; Envi-
Carb; Envi Carb/PSA
Comprehensive technical conditions were used by 30 Labs
6742
40586
22879
2193
8915
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
Results of target
pesticides
Monitoring ions
Ion abundance
Calibration curve
Results of pre-
collaborative study
Five types of data
30 laboratories have submitted to SD clusters of data totaling 81315
the method efficiency can be evaluated comprehensively with these five classes of data, and
errors can be analyzed and traced. In addition, it also includes a 5000-page of raw data reports
and chromatographs.
A lot of outliers obtained by one out of 30 laboratories were eliminated because of its digression from the
operation protocol, so this laboratory’s data was excluded from the evaluation of the method that ensued.