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SINGLE LABORATORY VALIDATION
4. In your experience with the method, do you have data demonstrating linearity, accuracy,
repeatability, LOD/LOQ?
Lab
Organization
Question 4
Lab-1
Asia pacific technicalcenter, the coca-cola company
N
Lab-2
Inspection & QuarantineTechnique Centre of Fujian Entry-exit Inspection and Quarantine Bureau
Y
Lab-3
HuangshanTea QualityAnd Safety Research Center of Huangshan Entry-Exit Inspection & QuarantineBureau
Y
Lab-4
Food inspection central of CIQ ShenZhen
Y
Lab-5
QinhuangdaoEntry-exit Inspection and QuarantineBureau
Y
Lab-6
Japan grain inspection association central research laboratory
N
Lab-8
Tea research institute ChineseAcademy of agricultural sciences
Y
Lab-9
EURL-FV, University of Almería
N
Lab-10
Dalianentry-exit inspection and quarantine bureau
Y
Lab-11
ICAS Testing Center (Shanghai)
Y
Lab-12
BENGBUCenter for Disease Control and prevention
N
Lab-13
Shanghai BinoTesting Service Co., Ltd
Y
Lab-14
DENO TESTING Service (Shanghai)
Y
Lab-15
Tea Research Institute, Chinese Academy of Agricultural Sciences
Y
Yes: 10 Labs No: 4 Labs
Lab -2:
The data obtained using AOAC Official Method 2014.09 to demonstrate the linearity, accuracy and
repeatability attached in Appendix (see Table below).
Lab-13:
Recoveries for 41 pesticides in green tea and black tea determined by GC-MS (see Table below).
No.
Pesticides
Retention
time/min
Green tea
Calibration curve
Spike
levels
(μg/kg)
Recovery/%
RSD/
%
R
2
Slope Intercept
No.1 No.2 No.3 No.4 No.5 AVE
IS heptachlor
18.787
1 trifluralin
9.940
0.9997
1.0858
-0.0155
200
82.2
85.5
83.7
84.7
94.2
86.1
5.47
2 tefluthrin
12.609
0.9982
1.8509
0.3906
100
88.1
89.7
87.6
87.3
97.3
90.0
4.66
3 pyrimethanil
14.156
0.9977
2.6666
0.5547
100
81.3
83.3
80.9
80.8
94.5
84.2
6.96
4 propyzamide
13.246
0.9984
0.9642
0.2457
100
83.8
85.5
80.3
81.5
87.2
83.7
3.37
5 pirimicarb
15.174
0.9981
1.5951
0.4131
100
91.6
94.2
83.1
91.0
103.7
92.7
7.99
6 dimethenamid
15.305
0.9963
0.4662
0.1679
40
86.7
95.2
90.8
89.8
102.3
93.0
6.52
7 tolclofos-methyl
16.264
0.9971
1.3229
0.3183
100
88.3
90.9
88.5
87.9
97.4
90.6
4.40
8 fenchlorphos
16.017
0.9965
1.2458
0.3545
200
95.1
99.2
95.4
96.7
103.6
98.0
3.62
9 pirimiphos-methyl
16.337
0.9977
0.5001
0.1095
100
90.1
92.0
89.7
89.1
98.1
91.8
4.01
10 2,4-DDE
20.000
0.9977
4.0213
0.7936
400
113.4
101.0
86.4
84.7
94.6
96.0
12.18
11 bromophos-ethyl
19.611
0.9973
0.3133
0.0691
100
90.5
92.3
89.6
89.4
99.1
92.2
4.36
12 4,4-DDE
21.820
0.9978
3.1875
0.6239
400
83.9
86.1
83.9
83.8
92.9
86.1
4.56
13 procymidone
19.907
0.9975
0.3892
0.0890
100
87.7
92.6
90.8
87.6
98.0
91.3
4.68
14 picoxystrobin
21.198
0.9979
0.7657
0.1335
200
88.1
91.4
87.6
87.7
95.2
90.0
3.68
15 chlorfenapyr
23.250
0.9976
0.4209
0.0998
800
97.2
99.1
95.9
95.4
102.6
98.0
3.01
16 quinoxyfen
27.183
0.9980
1.1099
0.2239
100
81.3
84.3
82.1
81.7
91.9
84.2
5.27
17 benalaxyl
26.357
0.9976
1.5987
0.3213
100
85.9
90.7
87.0
86.2
93.9
88.7
3.91
18 bifenthrin
28.293
0.9978
3.0963
0.8757
100
85.0
88.4
85.7
84.2
92.7
87.2
3.97
19 diflufenican
27.751
0.9982
2.0066
0.3759
100
86.3
89.8
86.5
85.2
94.3
88.4
4.20
20 bromopropylate
29.781
0.9978
1.8023
0.3328
200
87.0
91.5
88.5
87.3
95.5
90.0
3.99
Lab-2:
Determination results of multi-residues in tea by
GC-MS