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AOAC RESEARCH INSTITUTE
AOAC
OFFICIAL METHODS OF ANALYSIS
(OMA)
OMAMAN-13: Determination Of Dietary Starch In Animal Feeds And Pet Food By An Enzymatic-
Colorimetric Method Collaborative Study*
Study Director: Mary Beth Hall, U. S. Department of Agriculture – Agricultural Research Service, U.S.
Dairy Forage Research Center, 1925 Linden Drive, Madison, WI 53706, USA
First Action Recommendation
ER 1 Yes
ER 2
Yes, I do recommend the method to be adopted as First Action Method by ERP after authors have
explained the following two limitation of the method in application. 1. The method in the current
format is not be easily applicable to foods/feeds high in phenolic compounds (e.g. beets, red sorghum
grain). 2. Oats beta-glucan interfere in the assay and provide values above LOD = 0.31 +/- 0.09%.
ER 3
Yes. Recommend consideration of allowing HPLC as an option to measure liberated glucose to
calculate %dietary starch. This approach would also measure free, inherent glucose and track if
sucrose-digestion has occurred.
ER 4
Yes
ER 5
Yes.
ER 6
Yes
ER 7
yes
ER 8
Yes
After First Action Recommendation
ER 1 Use feedback
ER 2
NA
ER 3
Recommend consideration of allowing HPLC as an option to measure liberated glucose to calculate
%dietary starch. This approach would also measure free, inherent glucose and track if sucrose-
digestion has occurred. Decision needed if single or multiple lab work is needed to verify.
ER 4
Just the normal 2 year feedback period. Collaborative is completed and complete.
ER 5
N/A
ER 6
It would be good to test the performance of the method when an alternative glucose assay is used.
Clarify the reason why dextrin recovery is low.
ER 7
no
ER 8
That included in the text above. (Please clarify)