AOAC-RI ERP Book - FAOM METHOD.pdf

Page 3 of 8

Method Performance Feedback 2014.03

aromatic hydrocarbons in fish and shrimps integrated into a single methodOriginal Research Article

Pages 84-91

Kamila Kalachova, Jana Pulkrabova, Lucie Drabova, Tomas Cajka, Vladimir Kocourek, Jana Hajslova

analytica chimica acta

D. Reference Standards

NIST standard reference, Standard Reference Material (SRM) 2977 should be mentioned in the method.

The study, as reported in Mastovska et al.: Journal of AOAC International Vol. 98, No. 2, 2015 used

Reference Material 1974b, which is a mussel matrix with

certified concentrations of incurred PAHs and other organic

contaminants

NIST SRM 1974b was used for method qualification, but no data was presented.

Labeled PAHs:

Unlabeled PAHs:

PAH mixes:

There were certificates of Standard Reference Material (SRM) 2977 and Standard Reference Material (SRM)1974,

but no data were found from those reference materials to further demonstrate the method performance.

What type of single laboratory validations were used for this method [i.e, Harmonized PTM, Precollaborative,

Independent, Collaborative, etc.] and where is it referenced in the method documentation?

Collaborative, Performance characteristics on page 72 of the ERP book

A Collaborative Study was done. This is stated in the title of the document as well as in the abstract, and in the

document starting on page 479 of the publication

Collaborative

A full collaborative study was conducted and reported in Mastovska et al.: Journal of AOAC International Vol. 98,

No. 2, 2015

Collaborative study was conducted, with 10 labs successfully completing the validation process.

Precollaborative Independent,

Reference 1 in Mastovska et al.:J. AOAC Int., 98(2)2015.

Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Seafood Using Gas Chromatography-Mass

Spectrometry: Collaborative Study.

It was a collaborative study, which was published at J AOAC Int, to demonstrate the method performance.

Are the approved validation protocols available and documented?

Yes