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Michael Patrick Valley
Home Work
5463 Shale Road 2800 Woods Hollow Road
Fitchburg, WI 53711 Madison, WI 53711
(608) 332-3397 (608) 274-1181 ext 1533
Current Position
Senior Scientist I in the Assay Design Group of R&D at Promega
Technical Skills
 Design of various luminescent assays
 Mammalian cell culture, including various 3D cell culture methods
 Site-directed mutagenesis and subcloning of genes for overexpression in Escherichia coli
 Protein purification including anion exchange, hydrophobic, affinity, and size exclusion chromatographies
 Kinetics (steady state, stopped-flow, and rapid quench) including anaerobic work, isotope effects, and pH and
temperature dependencies
 Enzyme inhibition and substrate rescue studies
 Directed evolution of enzymatic activity including genetic knockouts in Escherichia coli
 UV/visible, fluorescence, resonance Raman, 1D NMR, and EPR spectroscopies
 Synthesis and purification of substrates
Research Experience
 Senior Research Scientist (November 2004 – present)
Research and Development, Promega, Madison, Wisconsin – design of luminescent assays to measure enzyme
activities, transcriptional reporters, and cellular viability.
 Postdoctoral Research Associate (June 2001 – October 2004)
Dr. Paul F. Fitzpatrick, Biochemistry and Biophysics, Texas A&M University, College Station, Texas -
chemical, kinetic, and structural characterization of the flavoenzyme nitroalkane oxidase .
 Graduate Student (August 1994 – May 2001)
Dr. John D. Lipscomb, Biochemistry, Molecular Biology and Biophysics, University of Minnesota,
Minneapolis, Minnesota - spectroscopy, mutagenesis and structure-function studies of the metalloenzyme
protocatechuate 3,4-dioxygenase.
 Industrial Internship (August – November 1998)
Dr. F. Sima Sariaslani, E.I. DuPont Central Research & Development, Wilmington, Delaware - protein
purification and characterization.
Education
 University of Minnesota, Minneapolis, Minnesota, Ph.D. in Biochemistry, Molecular Biology and Biophysics,
July 2002, GPA 4.0/4.0.
 University of Illinois, Champaign, Illinois, B.S. in Chemistry, May 1994, GPA 5.0/5.0, summa cum laude with
highest distinction.
 San Jose State University, San Jose, California, Department of Energy sponsored six week course in Nuclear
and Radiochemistry, Summer 1992.
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