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518

F

eng

et al

.:

J

ournal of

AOAC I

nternational

V

ol

.

100, N

o

.

2, 2017

Figure 10. E-gram of infant formula 1 with sweet whey ingredient (60% whey claim).

Figure 11. E-gram of infant formula 2 with sweet whey ingredient (60% whey claim).

Figure 12. E-gram of infant formula 3 with α-Lac-enriched whey ingredient (65% whey claim).

property of proteins and depends on the proteins’ amino acid

sequence and molecular structure status. Unfortunately, no

literature is currently available regarding whey protein and

casein ratios under SDS-CGE conditions, nor for proteins after

infant formula processing. In contrast, the mass ratio of whey

proteins to caseins is well established (4, 5; Table 8) and can be

calculated as 26.9% (whey proteins versus caseins) in bovine

milk and SMP.

To correct for the difference between the mass-to-area ratios

of whey proteins and caseins, 13 SMP samples from different

batches and suppliers were analyzed by SDS-CGE with 50

measurements. The two whey protein areas and the one casein area

were integrated, and the area percentage ratio of whey proteins to

caseins was established at 20.8% (Table 9).

The mass-to-area CF for whey proteins relative to caseins

was obtained by comparing the whey-to-casein mass percentage

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