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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
- Sucrose and monosaccharides are eliminated, avoiding their interference in the analysis.
- All components of the fructan are measured, thus there is no need for ingredient‐specific correction
factors.
-The method is sufficiently sensitive to detect low amounts of fructan.
- Only one
chromatographic run is needed.
Supporting Data
•
General Comment
:
-
Method Optimization:
•
Performance Characteristics
:
Does the method meet the SMPR for the following:
Analytical Range:
SMPR analytical range 0,03 – 5,0 %
NRC
0.03 – 5.0 g/100 g
CCC
0.03 – 5.0 g/100 g
LOQ:
SMPR ≤ 0,03%
NRC 0.018 g/100 g
CCC 0.016 – 0.030
Limits of detection and quantification were determined by analyzing a blank sample (KBI‐00052,
Infant formula Powder Soy Based) 14 times (7 days in duplicate). The sample has minor signals at
expected retention times of glucose and fructose.
Accuracy/Recovery:
SMPR 90 – 110%
NRC
86 – 119 %
CCC
89 ‐ 102 %
Of the 13 fructan‐free samples, 6 were selected for use in spike/recovery experiments. The 6 matrices were
spiked with 3 different fructans ingredients at three different levels.
T
hey were analyzed in duplicate on three different days by two different operators on two
different instruments without spiking (level 0) and spiked with Orafti HP, Orafti P95 or Nutraflora
P95, each at one of three levels .
At the 2 higher spiking levels recoveries were in general very good (92 – 104 %) with one exception, the
adult nutritional RTF high protein sample, for which the average recovery was only 86 %.
Precision (RSD
r
):
SMPR (%RSD
r
) ≤ 6%