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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
3
Analytical Range:
0.8-2250 ug/100 g – meets SMPR
LOQ:
For both lutein and β-carotene 0.27 ug/100 g (meets SMPR)
Accuracy/Recovery:
Accuracy by spike recovery at 2 levels of all-trans lutein and all-trans b-carotene were completed
for
of SRM1849a as well as 2 other matrices – meet SMPR for recoveries and repeatability (no
reference or certified carotenoid values available from NIST)
Precision (RSD
r
):
All SPIFAN matrices were analyzed and repeatability and intermediate reproducibility meet SMPR.
Only few matrices had repeatability higher than specified by SMPR abut these matrices had the analytes
present at concentrations below the analytical range specified by SMPR
Reproducibility (RSD
R
):
no data available
•
System suitability:
System suitability parameters are clearly defined. The method specifies how to
prepare system suitability mixtures of isomers and what resolution between them is expected
Recommendation:
I recommend this method as AOAC First Action method for
determination of lutein and β-carotene only in
infant formula and adult nutritionals providing the scope of the method is clearly defined.