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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
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Analytical Range:
2 – 4375 µg/100g
2 – 10,000 µg/100s with 2.3 g sample and subsequent dilution for samples > 4000 µg/100g
Not clear if SMPR is met
LOQ:
For total vitamin B
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(nicotinic acid + nicotinamide) = 195 µg/100g. Each analyte is therefore
<< 200 µg/100g. It would be beneficial to see the LOQ of each vitamin rather than a total.
SMPR met
Accuracy/Recovery:
Spikes at three levels, each in triplicate were 99.5% for nicotinic acid and 99.4% for
nicotinamide.
NIST 1849a averaged 107.2 µg/g (n = 4) representing 99.1% recovery against the certified value
of 108.13 ± 9.92 µg/g (mg/kg)
SMPR met
Precision (RSD
r
):
Eleven SPIFAN matrices were evaluated for precision (n=6) with the following RSR
r
:
3.5%, 5.4%, 5.4%, 6.0%, 6.0%, 5.8%, 5.0%, 5.0%, 5.6%, 5.5% and 5.1% respectively.
SMPR of ≤5% is not met in 8 of 11 samples.
Recommendation:
METHOD IS RECOMMENDED FOR FIRST ACTION
RSD
r
for the individual vitamins should be given to help decide why the repeatability was exceeded.
Can Takadiatase enzyme be omitted in future tests?
Postscript. The LOQ of the SMPR should be revisited in light of the Codex requirements for vitamin B
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