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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
Data for alpha-carotene and Lycopene were not collected
Method Clarity:
Method clearly written and should be easy to follow.
Method Safety Concerns:
No safety concerns are noted by the authors
Pros/Strengths:
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Well-established methodology
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Clearly written and easy to follow
•
Good chromatography for listed carotenoids
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Repeatability and recovery data for lutein and β-carotene meet SMPR
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Acceptable linearity
Cons/Weaknesses
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Not all carotenoids specified by SMPR are determined by this method. The authors are provided the
following explanation for exclusion of a-carotene and lycopene: “While alpha‐carotene is separated
with this method, it was not included in the determination because only one SPIFAN matrix contained
the analyte. Lycopene is also separated, but is excluded due to poor standard injection precision and
poor standard stability.
Even though validation data are not collected for α-carotene and lycopene both compounds were shown to
be separated by the present method from all lutein and β-carotene isomers
Supporting Data
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General Comment:
-
Method Optimization:
•
Performance Characteristics: