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Submission Date
2016-07-27 15:55:23
Name
Nour Eddine ES-SAFI
Organization
Mohammed V University in Rabat
Title of Method
Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts,
and Dietary Supplements by HPLC-UV: Single-Laboratory Validation
AOAC Candidate Method
Number (e.g. ALN-01)
TEA-01-02
Applicable SMPR
2015.014
Summary:
The method Tea-2 presents results dealing with the quantification of 7 catechins (C,
GC, EC, EGC, GCG, ECG, EGCG) and caffein in green tea raw material, powdered
extract and dietary supplements containing green tee extract. Quantitative analysis
was performed by RP HPLC separation and UV detection.
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
Yes. The investigated analytes are all indicated in the applicability of the AOAC SMPR
2015.014. In addition to caffein 7 catechins are concerned by the study and included
(+)-catechin, galloxcatechin, (-)-epicatechin, epigallocatechin, gallocatechin gallate,
epicatechin gallate and epigallocatechin gallate
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
LOQ: not clearly indicated.
LOD: not indicated.
Reproducibility: not conducted.
Recovery: Bad results were obtained in some cases.
Repeatability: Good results were obtained with EGC, caffein, EGCG. Bad results were
obtained with GC, C, EC, GCG and ECG.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
Yes
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
Yes
AOAC SPDS ERP - Set 3 Review Form 2