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Submission Date
2016-07-28 12:47:46
Name
Martha Jennens
Organization
Covance
Title of Method
Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts
and Dietary Supplements by HPLC-UV: Single Laboratory Validation
AOAC Candidate Method
Number (e.g. ALN-01)
TEA-02
Applicable SMPR
2015.014
Summary:
A rapid method to quantify seven catechins and caffeine in green tea raw material and
powdered extract and dietary supplements containing green tea extract. The method
utilizes RP HPLC with a phenyl column and gradient elution with UV detection and a 13
minute run time.
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
No. The method only applies to seven of eight catechins, one of three methyl
xanthines (caffeine) and no theaflavin or theanine is covered.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
Yes, but it does not cover all analytes in the SMPR.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
Yes
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
There is no discussion in the method around safety/precautions in use of chemicals,
etc.
AOAC SPDS ERP - Set 3 Review Form 2