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2. Does the method contain
system suitability tests or
controls as specified by the
SMPR? If not, please indicate
if there is a need for such
tests or controls and which
ones.
Yes the method contains system suitability test including duplicate injections of
standard solution and calibration standard.
3. Is there information
demonstrating that the
method system suitability
tests and controls as
specified in the SMPR worked
appropriately and as
expected? If no, please
specify.
Repeatability results obtained using the SRM 3254 reference material showed many
bad data. This is the case of GC (14.9 %), C (10.8 %), EC (5.16 %), GCG (5.72 %) and
ECG (5.66 %). The AOAC SMPR 2015.014 indicated teh obtained values should be
less or equal to 5.
Repeatability results with the SRM 3255 reference material showed good results.
Repeatability results with the SRM 3256 reference material showed good results
except for GC and C where bad values have been obtained (5.57 and 8.94 %
respectively).
4. Based on the supporting
information, is the method
written clearly and concisely?
If no, please specify the
needed revisions.
Yes
5. Based on the supporting
information, what are the
pros/strengths of the
method?
The proposed method is rapid, simple and robust. The optimized used
chromatographic conditions gave chromatographic profiles where the investigated
analytes are separated during a relatively short time.
6. Based on the supporting
information, what are the
cons/weaknesses of the
method?
Not all the method performance requirements are performed.
Does not meet the SMPR
7. Any general comments
about the method?
No
Do you recommend this
method be adopted as a First
Action and published in the
Official Methods of Analysis
of AOAC INTERNATIONAL?
Please specify rationale.
I do not recommend this method be adopted as a first action in the present form. I think
that more experiments are needed and should concern all the performance
requirements indicated in the 2015.014 SMPR.