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Biophysical Society 59
th
Annual Meeting, Baltimore, Maryland
email to
events@heka.com. The number of available spaces, food and drink
are limited, and registrations are accepted on a first-come-first-served basis.
Who should attend?
• Scientists with experience in patch clamp electrophysiology and
related scientific techniques
• Researchers who want to become more efficient in the use of
electrophysiology acquisition and analysis software
• PostDocs and graduate students who want to learn more about
electrophysiology techniques
Presenters
Hubert Affolter, Senior Software Architect, HEKA Elektronik
Christian Heinemann, General Manager, HEKA Elektronik
Telly Galiatsatos, General Manager, HEKA Instruments
Room A: Tuesday, February 10
1:30
pm
–3:00
pm
KinTek Corporation
KinTek Explorer Software: New Advances in Fitting Kinetic and
Equilibrium Data
Fitting kinetic data based upon numerical integration of rate equations
offers many advantages over conventional fitting of data based upon equa-
tions derived from simple models. Fitting by simulation is the most rigor-
ous and eliminates numerous errors in simplifying assumptions needed
to derive equations. Every day papers are published that contain errors in
kinetic analysis that could have been avoided if the data had been fit using
KinTek Explorer software.
In this presentation, Dr. Johnson will show how global fitting of kinetic data
can be accomplished with ease using the fast, dynamic simulation in KinTek
Explorer software, overcoming the all-to-common errors in conventional
fitting. Moreover, data are fit to derive rate constants directly defining steps
in a model, not merely observed rates (Eigenvalues). New advances in the
software allow fitting kinetic data from single molecule experiments and
families of curves can be fit simultaneous to define voltage-dependent rate
constants or data from Temperature-jump or Pressure-jump experiments.
In addition, equilibrium titration data can be fit using a unique endpoint
simulation method, and time-resolved spectra can be fit using singular
value decomposition (SVD). All experiments can be fit simultaneously and
accurate error estimates are derived using robust confidence contour analysis
Presenters
Kenneth A. Johnson, President, KinTek Corporation
Roger Williams, Professor of Biochemistry, University of Texas at Austin
Room B: Sunday, February 8
10:30
am
–12:00
noon
Carl Zeiss Microscopy LLC
Technology Innovations from ZEISS, the New ZEISS LSM 880
Confocal with Airyscan and the ZEISS Lightsheet Z.1
New microscopes from ZEISS address both ends of the spectrum of sam-
ples, live high speed imaging with superresolution and high speed imaging
of large live and fixed tissues. Learn how the ZEISS LSM880 with Airyscan
maintains the mantra that each photon of emission light is precious, while
expanding the triangle of sensitivity, resolution and speed of acquisition.
The LSM 880 with Airyscan allows you to use multicolor samples with any
label and get image quality like you’ve never seen before. With Airyscan you
are always able to select the optimal acquisition strategy for your sample:
Simply decide whether you want to gain 1.7x higher resolution in all three
dimensions – resulting in a 5x smaller confocal volume. Or push the sensi-
tivity beyond the limits of all conventional confocals. Or use the increase in
signal-to-noise ratio to speed up your image acquisition.
Traditionally, deeply imaging into intact tissue typically requires multipho-
ton excitation to penetrate deeper than near the surface of a tissue. Using a
“clearing” method to remove the light obstructing opaque molecules from
a tissue has been another technique for deep imaging. Techniques such
as SCALE, CLARITY, ClearT, SeeDB, CUBIC and others have allowed
researchers to image deeper than a millimeter into cleared animal model
brains and organs.
The ZEISS Lightsheet Z.1 features high speed image acquisition and greatly
reduced photodamage making imaging of live developmental samples and
fixed and cleared tissues easier than ever before. Come learn about using
the innovative ZEISS Lightsheet Z.1 microscope for imaging of fixed and
cleared tissues.
Presenters
Joseph Huff, Product Marking Manager, Laser Scanning and
Superresolution Microscopy, Carl Zeiss Microscopy LLC
Scott Olenych, Product Marketing Manager, Imaging Products, Carl Zeiss
Microscopy LLC