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187
Biophysical Society 59
th
Annual Meeting, Baltimore, Maryland
4:30
pm
–6:00
pm
Molecular Devices LLC
Eliminating 50-60 hz Line-frequency Noise with the New
HumSilencer and pCLAMP Software Tips &Tricks
We will introduce a new feature of the Axon DigidataTM 1550A digitizer,
HumSilencer, which provides a smart and simple method for eliminating
50 or 60 Hz line-frequency noise. In addition, we will present solutions
to frequently asked questions on our pCLAMP software, a powerful data
acquisition and analysis software that is used widely for a variety of electro-
physiological recordings in many academic laboratories.
Presenter
Jeffrey Tang, Axon Product Marketing Manager, Molecular Devices LLC
Room B: Tuesday, February 10
10:30
am
–12:00
noon
SensiQ Technologies Inc
Learn How SensiQ’s Dynamic Injection (diSPR®) Techniques Enhance
the Biophysical Characterization of Binding Events Using Surface
Plasmon Resonance Technology
SensiQ’s dynamic injection methods provide complete, one-pass kinetic
and equilibrium data from a single injection while reducing statistical error/
noise. Simply load one, highest analyte concentration vial and the instru-
ment exposes the surface to either a stepwise (FastStep®) or continuous
gradient (OneStep®) of concentrations. These approaches increase the ease/
throughput of SPR experiments and provide complete data sets for interac-
tions that are complicated by incomplete surface regeneration.
FastStep® uses a patented onboard micro-mixing technique to create increas-
ing fixed concentrations of analyte in real time without generating partial
dissociation responses as the instrument prepares subsequent concentra-
tions. This technique improves throughput by decreasing the time to com-
plete a full run while simplifying data analysis.
OneStep® is the ultimate evolution of FastStep®. Taylor dispersion fluidics
establish a continuous gradient of analyte concentrations which is flowed
over the surface to generate a sigmoidal binding curve. This technique
introduces a time dependent variable that is not possible in traditional
injection techniques and allows for the quantitative separation of multiple
binding sites with different affinities. OneStep® also increases the dynamic
range of allowable concentrations thereby removing the need to perform
test injections or accurately guess the affinity of an unknown interaction.
Importantly, OneStep® also provides added data content in SPR experi-
ments by providing a measure of the analyte diffusion coefficient to help
identify analytes that have a tendency to oligomerize or aggregate.
SensiQs’ operational software was developed to simplify assay development
and instrument operation. Using a drag and drop icon based program-
ming approach, traditional program “scripting” is eliminated to simplify and
speed assay development. Executable protocols for high throughput experi-
ments can be developed in minutes. Programming examples will show how
operational actions have been optimized to decrease runtime and increase
throughput. Streamlining data analysis of small or large data sets using our
Q-Dat software will also be presented.
Presenters
Derek Beahm, SensiQ Application Scientist
Rick Cope, SensiQ Sales Representative
12:30
pm
–2:00
pm
Nanion Technologies GmbH
Measure More Membrane: Cells, Bilayers and Transporter Activity
The Port-a-Patch turned 10 years old last year, and is going stronger than
ever. It’s still the smallest patch clamp rig in the world, and makes patch
clamp recordings accessible to anyone spending a couple of hours with
it. Giga-seal recordings and the excellent voltage-clamp of the cellular
membrane ensure high quality data, and the Port-a-Patch add-ons allow
unprecedented experimental freedom, including temperature control, inter-
nal perfusion, automated action potential recordings, and recordings from
primary and stem cell-derived cells.
The Orbit 16 is a parallel device for efficient formation of and recordings
from up to 16 artificial bilayers at once, for parallel bilayer-reconstitution of
ion channels and nanopores. Using Micro Electrode Cavity Array (MECA,
Ionera), a 4 x 4 array of circular micro-cavities in a highly inert polymer,
the bilayer is automatically formed by remotely actuated painting (Ionera-
SPREAD), which all will be demonstrated during the session.
Ion transporters and pumps play an important role within general metabo-
lism and information processing of organisms. The SURFE2R is a unique
platform for direct measurements ion transporters and ion channels in
diverse and heterologous membranes. It is easy-to-handle, highly sensitive
and a very efficient screening platform. The SURFE2R N1 is a small foot-
print, fully automated device recording from membrane preparations, with
proven success using native tissue, mammalian and insect cell lines, bacteria,
organelles, and proteoliposomes.
Join this workshop for hands-on experiments and information about three
outstanding platforms: Port-a-Patch, Orbit 16 and SURFE2R N1! We
look forward to seeing you!
Spaces are limited so reserve yours by sending an email to
info@nanion.de.
Presenters
Andrea Brüggemann, CSO, Nanion Technologies GmbH
Maria Barthmes, Application Specialist, Nanion Technologies
Gerhard Baaken, CEO, Ionera