Biophysical Society 59th Annual Meeting Program Guide

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186 Biophysical Society 59th Annual Meeting, Baltimore, Maryland
10:30 am–12:00 noon
Molecular Devices LLC
Performing Positive Allosteric Modulator (PAM) Assays and
Investigating Use-Dependent Inhibition of Ion Channels on
Automated Electrophysiology Systems Including the IonFluxtm
Benchtop Reader and the IonWorks Barracuda® Instrument
PAM Assays
Nicotinic acetylcholine receptors (nAChRs) have been extensively studied
due to their importance in physiological processes as well as involvement in
several muscle and neuronal human pathologies, and are major therapeutic
targets for pharmaceutical drug discovery. Ensemble recordings on the
IonFlux HT System were validated with human hnAChR recombinant cell
lines developed by Eurofins Discovery Services. Response properties of the
nAChRs to the endogenous ligand acetylcholine (ACh), reference agonists,
antagonists and positive allosteric modulators (PAMs) were characterized
and will be presented.
Ion Channel Use-Dependence
Use-dependent inhibition of ion channels by potential drug candidates is
an important aspect to investigate for many drug classes. Data will be pre-
sented to demonstrate the ability of automated electrophysiology systems to
study the use-dependence block of Na+ channel targets by peptide toxins
and known compounds. We will demonstrate the ability of the IonWorks
Barracuda system to deliver complex voltage protocols and generate long
assay windows which are required for these studies.Pulse trains delivered
at 10Hz are used to measure the blockade of current. These experiments
demonstrate stable assay windows with uniform currents for 30 minutes and
longer during the delivery of periodic pulse trains.
Presenter
James Costantin, Product Marketing Manager, Automated
Electrophysiology, Molecular Devices LLC
12:30 pm–2:00 pm
Nanion Technologies GmbH
HTS-Compatible Giga-Seal Ion Channel Drug Discovery: Beyond the
Bottleneck and Ready for CiPA
Nanion Technologies is one of the leading providers of automated patch
clamp systems, offering a diverse product portfolio covering a broad
experimental range from single channel recordings to HTS-compatible
ion channel screening from up to 768 cells in parallel. Allowing 20,000
data points per day, the SyncroPatch 384/768PE is unrivalled for high
throughput and high quality recordings. Diverse ion channel targets and
cell types have successfully been tested on the SyncroPatch 384/768PE
including challenging targets such as fast desensitizing ligand ion chan-
nels (P2X3 und GluA2), ion channels requiring intracellular activation
(Katp, TMEM16a) and heavily regulated channels such as TRPA1.
Early cardiac arrhythmic risk assessment is a hot topic these days calling
for new safety screening strategies. Patchliner, a medium-throughput APC
platform, supports automated current clamp recordings, experiments at
physiological temperatures, and a minimal cell usage, making it the ideal
partner for safety testing on stem cell derived cardiomyocytes. Additionally,
the CardioExcyte 96, a unique hybrid system for parallel impedance-based
and MEA-like recordings from intact cardiomyocyte networks, has proven
a versatile tool for safety and toxicity screening applications serving as an
excellent complement to APC. These three platforms enable you to keep up
with the requirements of the CiPA-initiative for early prediction of potential
cardiac arrhythmias.
During this workshop, we will show how to push the boundaries of ion
channel screening projects to achieve HTS-screening standards, and how to
get ready for comprehensive safety screening beyond hERG.
Spaces are limited so reserve yours by sending an email to .
Presenters
Niels Fertig, CEO, Nanion Technologies GmbH
Andrea Brüggemann, CSO, Nanion Technologies GmbH
2:30 pm–4:00 pm
Sutter Instrument
Scientists Empowering Scientists
For over 40 years, Sutter Instrument has designed and produced electro-
mechanical and optical instrumentation that helps scientists push the limits.
While Sutter has long been the market leader in products for micropipette
fabrication and micromanipulation, we have continued to expand our
Lambda imaging product line and XenoWorks microinjection systems. A
strong emphasis has always been placed on providing expert tech support to
help our customers achieve the best results in their research.
To further this goal, Sutter Instrument is starting a series of user meetings
with tutorial presentations. We will be providing step-by-step guidance to
the new experimenter as well as advanced tips and tricks for the experienced
user. To round it off, newly introduced products will be discussed on a
case-by-case basis.
Registration is available online through the Sutter Event Registration
Page ), or by email to . The
number of available spaces is limited, and registrations are accepted on a
first-come-first-served basis.
Who should attend?
• Electrophysiologists who use micropipettes and micromanipulators for
patch clamp, sharp electrode or extracellular recordings.
• Researchers who perform microinjections, including nuclear transfer,
sperm injection and application of substances into cell cultures or intact
organisms.
• Scientists who want to learn more about optimizing their results with
pipette pullers and micromanipulators
Presenters
Jan Dolzer, Tech Support and Product Development, Sutter Instrument:
Introductory Remarks
Adair Oesterle, Tech Support Micropipette Fabrication and Microinjection,
Sutter Instrument: Optimizing Settings on Your Sutter Micropipette Puller
Ali Mahloudji, Tech Support Micromanipulators and Lambda DG Series,
Sutter Instrument: Maximizing the Versatility of Your Dual-manipulator
Setup