Biophysical Society 59th Annual Meeting Program Guide

185

Biophysical Society 59

Annual Meeting, Baltimore, Maryland

12:30

–2:00

TA Instruments

Technology Advances in Ultrasensitive Isothermal Titration

Calorimetry

TA Instruments introduces the Affinity ITC, with all new technology for

advanced isothermal titration calorimetry. Isothermal Titration Calorimetry

is the most effective analytical tool for simply and accurately measuring

A/B interactions, especially protein-protein binding. Isothermal Titration

Calorimetry provides complete thermodynamics and kinetics without label-

ling, fixing, or otherwise altering the sample of interest. All new technology

from TA Instruments improves the sample throughput, usability, and data

quality of all isothermal titration calorimetry experiments.

All-new advanced stirring technology and an innovative isolated injection

system improves baseline stability, and mixing homogeneity while applying

minimal perturbation to the material of interest. For large-scale screening

and high throughput testing, an all-new unattended sample handling sys-

tem automates up to 96 full titrations and continuous unattended opera-

tion for multiple days. Based around a 96-well plate format and multiple

wash/rinse containers, the Affinity ITC Auto will greatly increase laboratory

productivity without sacrificing sensitivity or reproducibility. The Affinity

ITC is available in both the standard (1.0 mL) and low volume (190 µL) cell

sizes, extending the range of applications for which automation is available.

This presentation will include data examples and tech tips on experimental

design using the Affinity ITC Auto.

Presenter

Dile Holton, Microcalorimetry Product Manager, TA Instruments

2:30

–4:00

Bruker Nano Surfaces

Super-Resolution Microscopy and Its Applications in Fast and

Complex Biological Systems

Super-resolution microscopy has revolutionized the field of biological

imaging by providing new insights into biological processes in fields as

diverse as developmental biology, neuroscience, cardiovascular research,

genetics, infectious disease, and DNA/chromatin structure. The Vutara 350

super-resolution microscope offers a ten-fold improvement in resolution

in comparison to traditional light microscopy techniques and is capable of

achieving resolutions of 20 nm laterally and 50 nm axially. The Vutara 350

is based on a patented 3D biplane single molecule localization platform.

We will discuss the basic principles of operation and features of the Vutara

350 super-resolution microscope. The capability to do 3D multicolor

imaging, high speed live cell imaging, 3D particle tracking, and z-stacking

in various biological systems such as cells, tissue, drosophila, C. elegans,

bacteria and virus makes the Vutara 350 very versatile.

Presenter

Jeff Stuckey, Product Marketing Manager, Bruker Nano Surfaces

4:30

–6:00

OriginLab Corporation

Data Analysis and Graphing Using Origin 2015

Origin is an easy-to-use software application with data analysis and publica-

tion-quality graphing for science and engineering. This workshop will cover

key features including importing data frommultiple sources including Excel

and third-party file formats, LabVIEW connectivity, creating and custom-

izing multi-panel graphs, graphical exploration and analysis, curve fitting,

peak analysis, signal processing, and statistics. Time saving features such as

templates for graphing and analysis, batch plotting and batch analysis will

be presented. Application examples using Origin's programming environ-

ment will also be presented.

The workshop will also cover key new features and improvements in the

latest version:

Ease-of-use features including graph preview and comment tool tip in

Project Explorer, search for string in project, search for functions in dialogs,

redesigned axis dialog, enhanced legend, and custom categorical order. New

graph types including Heat Map, Kernel Density Plot, Column Scatter

Plot. Improvements to profile plot, box plot, contour plot, bubble scale,

and color scale. New analysis tools for Distribution Fit, ANOVA of unbal-

anced data, t-Test on rows. Tool to append worksheets, remove or combine

duplicate values, and improved pivot table. Integration of Python as script-

ing language in Origin.

Presenter

Easwar R. Iyer, VP of Technology, OriginLab Corporation

Room B: Monday, February 9

8:30

–10:00

FEI Company

Advances in Correlative Light and Electron Microscopy

Correlative light and electron microscopy (CLEM) is a powerful approach

that enables combining dynamic information and labelling specificity

from fluorescence microscopy with ultra-structural information at nano-

meter resolution from electron microscopy on the same sample. In recent

years technical improvements in fluorescence microscopy have enhanced

z-resolution, enabled imaging with high sensitivity using TIRF and, with

super-resolution microscopy, improved the resolution of light microscopy

to up to 20 nm. Despite all these advances, fluorescence microscopy can

only show what was labelled and an EM is needed to provide the full

morphological context on the ultra-structure of the cell. However, CLEM

experiments still remain challenging and low through-put.

Over the last years, FEI has introduced different solutions to overcome some

of the challenges in CLEM experiments and to make CLEM experiments

easier and more efficient. But correlative experiments are rapidly evolving –

here, we will present updates on latest developments that have pushed the

boundaries of correlative experiments.

Presenter

Meike Pedersen , Product Application Specialist, FEI Company