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Dust Characterization
•
Dust has been checked for cross reactive signatures with
MRI QA assays (LF, CapB, DHP7, chromosomal marker H1)‐
non‐reactive
•
Dust was checked for inhibition effects‐not inhibitory at
0.1 g using MRI extraction method
•
LOD of spiked
B. anthracis
in dust extract is 600 genome
equivalent copies (LF assay,)
•
Dust spiked with 600 spores tested positive for target (Ct=
36.7±0.45, N=50)
Reference Dust (SRD)
Reference Dust
Dust Application Device
•
Dust was pooled and dispensed into
244
×
50g aliquots. Aliquots were
lyophilized, labeled and stored.
0.1g ±0.023g for more
than 300 filters