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Soils
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Soils were collected from five geographic locations
across the United States
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Washington DC/Silver Spring
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El Paso
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Houston
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Seattle
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Kansas
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Not all sites had all types of soils (loam, sandy loam,
sand, clay, subsoil)
Soil Characterization
S il
if d
d b i
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o s were s te to remove e r s
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Tested for PCR inhibition extract from a 1 g extraction
of soil‐ spiked with 1 pg; Spike blank N=1; Ct=34.9, soil
extract N=24; Ct=34.4±0.23
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No signature cross reactivity in the soils using MRI QA
PCR reagents for
Bacillus anthracis
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DNA sent to University of Houston for Metagenomic
sequencing
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Soils analysis characterization was completed
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Soils were blended to make Master Lots