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“DIRECT” ANALYSIS METHOD
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
March17,2015
MFA‐AOAC,2015
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LC‐MS CONDITIONS
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The FDA publication used:
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RP C 18 + ion pairing conditions (TBA)
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APCI conditions, negative ionization mode
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Single MRM transition
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Our approach
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Multi mode ODS column +non ion pairing conditions
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ESI, negative ionization mode
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
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Two MRM transitions
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Summation of two transitions for quantitation
March17,2015
MFA‐AOAC, 2015
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