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LC CONDITIONS
Mobile Phase A: 0.1% formic acid in water
Mobile Phase B: 0.2% formic acid in methanol
Chromatography
Flow Rate
350 µL/min
Column Temperature
40°C
Injection Volume
5 μL
Sample Temperature
Ambient
Gradient Program
Time
% A1
% B1
Curve
0.00
100
0
6
2.00
100
0
6
6.00
2
98
6
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
March17,2015
MFA‐AOAC, 2015
13
9.00
2
98
6
9.01
100
0
6
11.00
100
0
6
MS CONDITIONS
MS Tune
Ionization Mode
ESI‐
Cone Gas
250 L/Hr
Capillary
2.5 kV
Nebulizer
7.0 bar
Source Offset
30.0 V
Collision Gas Flow
0.15 mL/min
Source Temperature 150°C
Quad 1 Resolution
Unit Mass (0 75 Da FWHM)
.
Gas Temperature
350°C
Quad 2 Resolution
Unit Mass (0.75 Da FWHM)
Desolvation Gas Flow 900 L/Hr
MS Calibration Range
50‐2000 amu
MS/MS Transitions
Compound
Parent Mass (m/z) Daughter Mass (m/z)
Dwell (s) Cone (V) Collision Energy (V)
MFA 1
77.0
33.0
0.250
20
10
MFA 2
77.0
57.0
0.250
20
10
MFA IS 1
81.0
36.0
0.250
20
10
MFA IS 2
81.0
60.0
0.250
20
10
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
March17,2015
MFA‐AOAC, 2015
14