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LC CONDITIONS
•
Waters Acquity ACQUITY UPLC
®
BEH C18 1.8 µm, 2.1 x 100 mm
column
•
Mobile phase A: 10 mM ammonium acetate in water
-
•
Mobile phase-B: 10 mM ammonium acetate in 95/5 (vol.)
acetonitrile/water
•
Gradient conditions
Chromatography
Flow Rate
300 µL/min
Column Temperature
40°C
Injection Volume
10 μL
S l T
A bi
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
March17,2015
MFA‐AOAC, 2015
23
amp e emperature
m ent
Gradient Program
Time
% A
% B
Curve
0.00
90
10
6
1.00
90
10
6
7.00
45
55
6
9.00
0
100
6
9.01
90
10
6
12.00
90
10
6
MS CONDITIONS
MS Tune
Ionization Mode
ESI‐
Cone Gas
250 L/Hr
Capillary
0.5 kV
Nebulizer
7.0 bar
Source Offset
20.0 V
Collision Gas Flow
0.15 mL/min
Source Temperature 150°C
Quad 1 Resolution
Unit Mass (0.75 Da FWHM)
Gas Temperature
350°C
Quad 2 Resolution
Unit Mass (0.75 Da FWHM)
Desolvation Gas Flow 900 L/Hr
MS Calibration Range
50‐2000 amu
MS/MS Transitions
Compound
Parent Mass (m/z)
Daughter Mass (m/z)
Dwell (s)
Cone (V)
Collision Energy (V)
MFA‐2NPH
212
182
0.15
20
15
MFA‐2NPH_IS
216
186
0.15
20
15
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
March17,2015
MFA‐AOAC, 2015
|
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