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CHROMATOGRAPHY
•
Multimode ODS column:
•
Scherzo SS-C18 column (100 x 3 mm)*
•
Purified Porous Silica / Particle Size 3um / Pore Size 13nm / ODS
+ Anion Exchange + Cation Exchange Ligands
•
Separation achieved through a manipulation of eluent pH, organic
solvent composition, salt concentration, and temperature.
•
Enables the retention of ionic species with LC-MS compatible
mobile phases
•
Avoids the use of ion pairing agents in mobile phases
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
•
Provides simple and rugged chromatographic retention
*= Imtakt USA, Portland, OR
March17,2015
MFA‐AOAC, 2015
11
SAMPLE PREPARATION
•
For powders:
–
Weigh 10.0
0.5 g of powder into a tared vessel and record mass.
–
Add water to a total weight of 70
5 g (powder + water) and record
mass.
–
Stir until homogenous.
•
For ready-to-feed liquids and reconstituted powders:
–
Weigh 5.00
0.20 g liquid into a tared 15 mL centrifuge tube and
record mass.
–
Add 40
L of internal standard working solution and vortex.
–
Add 50 μL of glacial acetic acid and 5.0 mL methanol, vortex.
–
Transfer aliquot to microfuge tube and centrifuge at 13,000 rpm for
Proprietaryand confidential — do not distribute
© 2015 Abbott Laboratories
10 min.
–
Filter an aliquot of the supernatant into an autosampler vial using a
0.2 um PTFE syringe filter.
March17,2015
MFA‐AOAC, 2015
12