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Amino Acids in Infant Formula and Adult/Pediatric Formulas
Ultra High Performance Liquid Chromatography
4
room temperature and heat in a preheated water bath at 55
˚C for 5
minutes. Final
concentrations of calibration standards are 1.0, 5.0, 10.0, 25.0 and 50.0 μM respectively.
3) Preparation of calibration standards for Tryptophan (alkaline hydrolysis method):
Table 4:
Stock std. Conc.
(mg/L)
Vol. of stock
Std. (mL)
Makeup Volume
(mL)
Calibration std.
Conc.(mg/L)
500.0
1.00
10.0
50.0
500.0
0.50
10.0
25.0
500.0
0.20
10.0
10.0
50.0
1.00
10.0
5.00
50.0
0.20
10.0
1.00
1.0
1.00
10.0
0.10
1.0
0.20
10.0
0.02
Amino-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE