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Amino Acids in Infant Formula and Adult/Pediatric Formulas

Ultra High Performance Liquid Chromatography

4

room temperature and heat in a preheated water bath at 55

˚C for 5

minutes. Final

concentrations of calibration standards are 1.0, 5.0, 10.0, 25.0 and 50.0 μM respectively.

3) Preparation of calibration standards for Tryptophan (alkaline hydrolysis method):

Table 4:

Stock std. Conc.

(mg/L)

Vol. of stock

Std. (mL)

Makeup Volume

(mL)

Calibration std.

Conc.(mg/L)

500.0

1.00

10.0

50.0

500.0

0.50

10.0

25.0

500.0

0.20

10.0

10.0

50.0

1.00

10.0

5.00

50.0

0.20

10.0

1.00

1.0

1.00

10.0

0.10

1.0

0.20

10.0

0.02

Amino-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE