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Amino Acids in Infant Formula and Adult/Pediatric Formulas
Ultra High Performance Liquid Chromatography
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f) Calculations:
Area of std. * Conc. of ISTD in μM
Relative Response factor (RRF) =
ISTD Area * Conc. of std. in μM
(A
s
- A
b
) * W
AA
* C
ISTD
* V
s
* DF
S
Amino acid (g/100g) =
RRF * A
ISTD
* 1000 * W
s
* 10000
A
s
= Sample area
A
b
= Blank area
W
AA
= Molecular weight of corresponding amino acid.
A
ISTD
= ISTD area of sample.
C
ISTD
= Conc. of ISTD in μM
V
s
= Sample Makeup Volume
W
s
= Sample weight in grams
DF
S
= Sample dilution factor (Other than V
s and
W
s
)
1000 = Conversion factor for μM to mM
10000 =Conversion factor for mg/kg to g/100g
G.
References:
a)
UPLC Amino acid analysis solution system guide. Waters
b)
ISO 13904:2005(E) : Animal Feeding stuffs
–
Determination of Tryptophan content.
c)
ISO 13903:2005- : Animal Feeding Stuffs
–
Determination of Amino acids Content.
d)
Sulfur amino acids in food, feed ingredients and processed foods
–
AOAC 985.28
e)
Amino acids in Feeds
–
AOAC 994.12
Amino-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE