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Amino Acids in Infant Formula and Adult/Pediatric Formulas
Ultra High Performance Liquid Chromatography
7
Column
ACCQ-TAG ULTRA C18 1.7μM, 2.1 x 100mm
Mobile phase A
Mobile Phase A1
Mobile phase B
Mobile Phase B1
Flow rate
0.6 mL/minute
Injection Volume
1.0 μL
Column Temperature
50 °C
Detector Wavelength
260 nm
Run Time
14 minutes
Gradient Method
Time (minutes)
A %
B%
0.00
99.90
0.10
0.63
99.90
0.10
2.33
99.50
0.50
2.92
98.70
1.30
3.50
98.00
2.00
4.12
97.00
3.00
4.70
96.00
4.00
5.64
93.90
6.10
5.99
93.10
6.90
6.16
92.60
7.40
6.39
92.00
8.00
6.70
90.90
9.10
9.03
78.80
21.20
10.00
40.40
59.60
10.01
10.00
90.00
11.01
10.00
90.00
11.02
99.90
0.10
14.00
99.90
0.10
3)
Chromatographic conditions for Alkaline Hydrolysis method:
Table 8:
Column
Agilent Zorbax RRHD, 1.8μm, 2.1 X 50 mm
Mobile phase
Mobile Phase A2
Flow rate
0.2 mL/minute
Injection Volume
2.0 μL
Column Temperature
25°C
Detector Wavelength
Ex. 280nm, Em. 356nm
Run Time
10minutes
Pump Mode
Isocratic
Amino-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE