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Table 2

Accuracy - Spike Recovery

Avg (%) n=3

RSD %

Avg (%) n=3

RSD (%)

Child Formula Powder, placebo

00847RF00

Biotin

6

Not detected

103

3.2

105

1.5

Adult Nutritional RTF High Protein, placebo

00821RF00

Biotin

6

Not detected

102

1.6

103

1.5

Adult Nutritional RTF High Fat, placebo

00820RF00

Biotin

6

Not detected

102

2.2

104

1.7

Infant Formula Powder Partially Hydrolyzed Soy Based

410457651Z

Biotin

6

4.43

105

4.2

101

4.2

Adult Nutritional Powder Low Fat

00859RF00

Biotin

6

31.9

111

2.9

102

1.8

Child Formula Powder

00866RF00

Biotin

6

21.6

109

2.8

104

1.7

Infant Elemental Powder

00795RF00

Biotin

6

10.7

106

2.8

95.1

3.0

Infant Formula Powder FOS/GOS Based

50350017W1

Biotin

6

1.66

105

6.4

99.9

7.2

Infant Formula Powder Milk Based

K16NTAV

Biotin

6

5.11

104

6.1

103

4.6

Adult Nutritional RTF High Protein

00730RF00

Biotin

6

56.8

109

1.6

102

0.8

Adult Nutritional RTF High Fat

00729RF00

Biotin

6

76.2

109

2.4

101

0.7

Global Mean %

106

3.3

102

2.6

Level 2 (~150% for placebo, ~100% for fortified)

Spike Recovery

Product

Nr. replicates (n)

Native level

(mcg/100g

liquid)

SPIFAN Code

Analyte

Level 1 (~50%)

SPIFAN ERP Checklist v1.0

Bio-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE