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Acq. Operator : Seq. Line : 6

Acq. Instrument : UPLC-DAD Location : P1-D-03

Injection Date : 7/6/2015 10:06:50 PM Inj : 1

Inj Volume : 1.000 μl

Acq. Method : D:\UPLC\DATA\COE\2015\JULY2015\04AMINOACIDS-VAL-2\04AMINOACIDS-VAL2 2015-07

-06 20-53-07\V-AMINOACIDS-1.M

Last changed : 7/6/2015 8:53:08 PM

Analysis Method : D:\UPLC\DATA\COE\2015\JULY2015\AA-VAL1\V-AMINOACIDS-1.M (Sequence Method)

Last changed : 8/1/2015 2:35:37 PM

(modified after loading)

Additional Info : Peak(s) manually integrated

min

2

4

6

8

10

mAU

0

5

10

15

20

25

30

35

DAD1 A, Sig=260,4 Ref=off (D:\UPLC\DATA\COE\2015\JULY2015\AA-VAL1\_006_AMINO ACIDS MIX-5UM-SS1.D)

2.295

3.030

3.298

3.475

3.604

3.937

4.450

4.863

5.248

Area: 18.0406

5.860

Area: 16.6504

6.796

Area: 27.5474

6.878

Area: 19.1716

6.988

7.182

7.361

7.525

8.372

8.489

8.632

=====================================================================

Internal Standard Report with Performance

=====================================================================

Calib. Data Modified : Saturday, August 01, 20152:35:11 PM

Multiplier : 1.0000

Dilution : 1.0000

Do not use Multiplier & Dilution Factor with ISTDs

Signal 1: DAD1 A, Sig=260,4 Ref=off

RetTime k' Sig Amount Symm. Width Plates Resol Name

[min] [μM] [min] ution

-------|------|---|----------||-----|-------|-------|-----|----------

2.295 - 1 0.00000 0.79 0.1067 2564 - Histidine

3.030 - 1 0.00000 0.71 0.0858 6894 4.49 Taurine

3.298 - 1 0.00000 0.76 0.0779 9937 1.92 Serine

3.475 - 1 0.00000 0.83 0.0702 13563 1.40 Argenine

3.604 - 1 0.00000 0.75 0.0793 11437 1.01 Glycine

Sample Name: Amino acids Mix-5uM

UPLC-DAD 8/1/2015 2:35:40 PM

Page 1 of 2

Amino-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE