549 / 670
Memo
Monique Steegmans
16001n-MSS-MSS-SLV FOS01 AOAC997.08 Beneo-Orafti, 1 Febr 2016, page 11/18
3.2 Accuracy
The standard performance requirement 2014.002 stated that the recovery ranges between 90 and
110% over the range of the essay. This recovery was measured by spiking 9 different matrices at
different levels covering the proposed range from 0.05 to 5 g/100g reconstituted product.
The 3 raw materials available in the Test Materials Kit were used for this spiking.
Product
Spike Recovery
SPIFAN
CODE
spike
level Native
level *
*
Average
(%)
RSD
%
Spiked with
SRM 1849a
CLC10-b
5
<0,03
98,2
1,7 Orafti P95
infant powder
partially hydr Milk
based
1172572116
1
<0,03
99,8
0,4 Nutraflora P95
infant powder
partially hydr Soy
based
117257651Z 0,05
<0,03
101,4
1,7 Orafti HP inulin
Adult nutr powder
low fat
00394RF00 0,05
<0,03
107,1
7,2 Nutraflora P95
Infant elemental
powder
00403RF00
1
<0,03
101,5
3,5 Orafti P95
Infant formula powder
Milk based
D04HTCVV
5
<0,03
96,9
1,6 Orafti HP inulin
Infant formula powder
Soy based
E29JVLV
5
<0,03
98,4
5,5 Nutraflora P95
Infant formula RTF
Milk based
SPIFAN
Control milk
form
EV4H2R
0,05
<0,03
100,8
2,3
Orafti P95
Adult nutr RTF high
Protein
00730RF00
1
<0,03
98,8
1,2 Orafti HP inulin
Average
100.3
*Fructan results are expressed in g/100 g reconstituted final product.
The average recovery for the different levels of spiking ranges from 96.9 to 107.1%, for which
107.1% seems to be a higher value in the series of recovery results. It is clear that this value is for
spiking at the lowest level which generates the highest error but for this sample were also amounts
of fructans observed but below the LOQ.
The RSD ranges from 0.4 to 7.2%, for which the highest value is also the lowest spiking level.
The overall average recovery is very well, being 100.3% and meets the requirements of the SMPR
2014.002.
Detailed results can be consulted in annex 5.
Fos-01 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE