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Memo

Monique Steegmans

16001n-MSS-MSS-SLV FOS01 AOAC997.08 Beneo-Orafti, 1 Febr 2016, page 7/18

Fructan = (Fi+Gi)*k2

Where k2 is a correction for the water gain during hydrolysis:

k2 = (180+162*(DP-1))/(180*DP)

DP is Degree of polymerisation

2.3 Validation

2.3.1 Precision.

All samples of the Test Materials Kit were screened for the presence of fructans. Four samples

were found to contain amounts above the LOQ of 0.03 g/100 g reconstituted product.

Adult nutr powder Milk Protein based

11750017V3

J4G-00019

Adult nutr RTF high Fat

00729RF00

J4G-00980

Child formula powder

00411RF00

J4G-00013

Child formula powder

00412RF00

J4G-00974

These samples were analyzed in duplicate, on 6 different days and by different operators. As the

method is consisting of different steps, the hydrolysis, silylation, integration were performed by

different people. The Relative Standard Deviation (repeatability conditions) (RSDr) and RSD(ir)

(intermediate precision) were calculated from the obtained data. Results are found under point 3.1

and in annex 4.

2.3.2 Accuracy.

For the accuracy, a spiking was done by the 3 different raw materials provided by the Test

Materials Kit.

Nutraflora P95 (FOS)

J4G-00010

Orafti P95 (FOS)

J4G-00011

Orafti HP (inulin)

J4G-00012

The Nutraflora and Orafti P95 are fructo-oligosaccharides with DP ranging from about 2 to 8.

Nutraflora is produced from sugar as raw material while Orafti P95 is originating from chicory roots.

The mean DP is 4.

The Orafti HP is a long chain inulin extracted from chicory with DP ranging from 2 until 60 and a

mean DP of min 23. The DP for this inulin HP (J4G-00012) was found to be 34.

The raw materials as included in the Test Materials Kit were analyzed by 2 different

chromatographic techniques being GC and HPLC. No significant differences in fructan content

between the applied techniques could be observed.

Those techniques are preferred above HPAEC as this last one needs enhanced calibrations as

Fos-01 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE