7
The thiachrome reaction was developed in 1935
for the analysis of thiamine in biological matricies
Thiachrome at pHs above 8.0 has a strong blue
fluorescence (ex 375nm, em 432–435nm)
Thiachrome analysis has been used for most of
the analytical data available for the B
1
content in
food.
Thiachrome is used as the determinative step in
the official methods of the USP, AOAC, and Food
Chemical Codex as well as many others
The Dietary Reference Intake (DRI), or daily requirement, for
Vitamin B
1
(Thiamine) is as follows:
children 0‐5 months
0.2 mg/day
children 6‐11 months
0.3 mg/day
children 1‐3 years
0.5 mg/day
children 4‐8 years
0.6 mg/day
children 9‐13 years
0.9 mg/day
males 14 and older
1 2 mg/day
.
females 14‐18
1.0 mg/day
females 19 and older
1.1 mg/day
During pregnancy and lactation 1.4 mg/day.
