Stakeholder Panel Meeting Book - September 6, 2014 - page 75

AOAC SMPR 2014.XXX; Version 2; March 19, 2014 1
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Method Name: Determination of Selected Carotenoids in Infant and 3
Adult/ Pediatric Nutritional Formula 4
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Approved by: Stakeholder Panel for Infant Formula and Adult Nutritionals
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Final version date:
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Effective date: 8
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Intended Use: Reference method for dispute resolution.
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1. Applicability: 12
Determinations of all -trans α -carotene (CAS 7488-99-5), cis isomers of α-carotene,
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all-trans β-carotene (CAS 7235-40-7), cis isomers of β-carotene, all-trans lutein (CAS
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127-40-2), cis isomers of lutein, and lycopene in all forms of infant, adult, and/or
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pediatric formula (powders, ready-to-feed liquids, and liquid concentrates).
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2. Analytical Technique: 18
Any analytical technique that meets the following method performance
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requirements is acceptable.
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3. Definitions: 22
Accuracy1
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The closeness of agreement between the average of an infinite number of replicate
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measured quantity values and a reference quantity value.
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Adult/Pediatric Formula
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Nutritionally complete, specially formulated food, consumed in liquid form, which may
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constitute the sole source of nourishment, made from any combination of milk, soy,
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rice, whey, hydrolyzed protein, starch, and amino acids, with and without intact
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protein.
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α-Carotene (all-trans alpha-Carotene)
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CAS number: 7488-99-5. A carotenoid with a β -ring at one end and an ε-ring at the
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opposite end. Figure 1.
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β-Carotene (all-trans beta-Carotene)
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CAS number: 7235-40-7. A symmetric carotenoid with β-rings at both ends of the
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molecule. Also known as beta,beta-carotene. Figure 2.
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Carotenoids
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A class of organic pigments consisting of four 10-carbon terpene units, which in turn
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are formed from eight 5-carbonisopreneunits. For the purposes of this standard,
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only the four carotenoids listed in the Applicability section are considered biologically
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important enough for consideration in this standard for in infant formula/adult
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nutritionals.
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Infant formula
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Breast-milk substitute specially manufactured to satisfy, by itself, the nutritional
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requirements of infants during the first months of life up to the introduction of
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appropriate complementary feeding2, made from any combination of milk, soy, rice,
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whey, hydrolyzed protein, starch, and amino acids, with and without intact protein.
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1 Corresponds to the VIM definition for “tru eness”.
2 Codex Standard 72 – 1981.