*
μ
g /100 g reconstituted final product.
87
88
5.
System suitability tests and/or analytical quality control:
89
Suitable methods will include blank check samples, and check standards at the
90
lowest point and midrange point of the analytical range. Methods must be capable of
91
resolving lutein from zeaxanthin.
92
93
6.
Reference Material(s)
: Neither NIST nor IRMM produce a certified reference material
94
for carotenoids in infant formula. The carotenoid content of SRM 1849a has not been
95
determined (as of 11/20/2013).
96
97
7.
Validation Guidance
:
98
Recommended level of validation:
Official Methods of Analysis
SM
.
99
100
8.
Maximum Time-To-Result
: No maximum time.
101
102
103
Figures:
104
105
106
107
Figure 1: Molecular structure of all-
trans
α
-carotene
108
109
110
111
112
Figure 2: Molecular structure of all-
trans
β
-Carotene
113
114
115
116
117
118
119
120
121
122
Figure 3: Molecular structure of
all-
trans
lutein.
123
124
125
126
127
128
129
Figure 4: Molecular structure of lycopene.
130
131
