Preferred analytical methods for speciation
Separation of species by chromatography using either
ion chromatography (IC) or high performance liquid
chromatography (HPLC)
Detection of the separated species by ICP: ICP‐MS is
the most common instrument and ICP‐MS has options
in equipment to obtain MS selectivity to minimize
i t f n er erences
IC‐ICP/MS or LC‐ICP/MS
Existing methods for speciation in food
Many papers and methods describing the same type of analysis but the
question is: “Are they robust methods when generalized to other foods?”
A il bl P bli h d th d A i
d M S i ti
va a e u s e me o s on rsen c an ercury pec a on:
Element
Method name
Technique
Published methods
Arsenic
Arsenic speciation in rice
LC-ICP-MS
US FDA : EAM 4.11
Arsenic speciation in juice
LC-ICP-MS
US FDA : EAM 4.10
Arsenic speciation in water
LC-ICP-MS, Nitric Acid mobile
phase
USGS I‐2193‐02
Arsenic speciation in water
LC-ICP-MS, Malonate/ Acetate
mobile phase
USGS I‐2192‐02
Methylmercury in water
Ethylation, CV‐AFS
US EPA: 1630
Methylmercury and total mercury in seafood
HPLC ICP MS
US FDA: EAM 4 8
Mercury
‐ ‐
.
Methylmercury in seafood
Extraction, elemental
analysis
EU IRMM IMEP-115
Mercury speciation in soils, sediments
Selective extraction
US EPA: 3200
Methylmercury in water
Ethylation, GC‐AFS
USGS OFR_01-445
Metal species in water, waste extracts
speciated IDMS
US EPA: 6800
