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•
ELISA and qPCR useful for peanut detection and quantification
•
Quantities obtained by qPCR in rice might be slightly more accurate
(more true compared to the expected/theoretical values) than
quantities obtained by ELISA
•
Kit to kit
variation
•
Matrix to matrix
variation:
Baked cookies, cumin powder less applicable matrices in this study
(influence of
processing
)
Effect of mixing flour to other cookies ingredients and the effect of
high-temperature baking clearly observed, in terms of less detection
and quantification in both ELISA and qPCR
This effect was even stronger for qPCR compared to ELISA
•
Variation
between the two labs
who used exactly the same test
materials for exactly the same set of 11 peanut test kits
Belgian NRL-Allergens
(2 labs + Federal Food Safety Agency)
Validation study on peanut detection -
Conclusions
Contribution from I. Taverniers
(ILVO) & N. Gillard (CER)
Factors influencing ELISA measurements
Antibodies
Monoclonal or polyclonal
Target of the antibody: single/several proteins, fractionated,
modified, synthesized
Material used for immunization