ISPAM Advisory Panel-Food Allergen

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6/14/2016
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Immunological test system
 Environmental influences
 Experimental conditions
 Stability affected by transport & storage conditions
 Robustness vs ruggedness
Factors influencing ELISA comparability
Summary
 Food allergens are extremely diverse
 Significant variability of results between
immunoassay methods, labs, matrices
 No certified reference standard
 no common reference point for ELISA comparison
 Many matrix factors influence accuracy – take
into account when choosing matrices
 Consider both inclusivity and exclusivity when
testing cross reactors
 Consider both robustness and ruggedness