6/14/2016
8
Immunological test system
Environmental influences
Experimental conditions
Stability affected by transport & storage conditions
Robustness vs ruggedness
Factors influencing ELISA comparability
Summary
Food allergens are extremely diverse
Significant variability of results between
immunoassay methods, labs, matrices
No certified reference standard
no common reference point for ELISA comparison
Many matrix factors influence accuracy – take
into account when choosing matrices
Consider both inclusivity and exclusivity when
testing cross reactors
Consider both robustness and ruggedness