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Cross reactivity
Positive response to a sample without target allergen
Test products genetically similar to target allergen
Different response than in study possible:
- raw vs. processed product
- variations within globally used cultivars
Factors influencing ELISA comparability
Cross reactivity
Choose appropriate cross reactivity
panel based on:
•
Genetic closeness
•
Tree of life closeness
•
Search for conserved domains
Panel should not only be exclusive, but
also inclusive:
•
Bovine/sheep/goat milk reactivity
•
Gliadin/hordein/secalin reactivity
Consider cross reactor panel choices
from international perspective
Factors influencing ELISA comparability