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Matrix Choices: Allergen Material
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Incurred
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Better option – allergen processed along with food
matrix
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True evaluation of extraction efficiency
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Difficult to design/predict allergen levels precisely
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Challenge for method developer
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Some incurred RM available for purchase
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No CRM
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Reasonable goal could be one incurred RM per
allergen for validation of trueness
Matrix Choices: Allergen Material
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Spike
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Necessary when validating more than a
couple matrices
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Necessary for certain matrices which cannot
be produced in lab
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Generally recover close to 100% of spike
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Useful for measuring precision, interference
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Allergen extract vs whole allergen
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Homogeneity