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What are we looking for?
Identify the target:
Raw protein or processed commodity?
Choose antibodies accordingly
Similar calibrators
SMPR’s can harmonize allergen methods, but
will be most effective if allergen reference
standards identified
Factors influencing ELISA measurements
Calibrators
Match the antibodies of the kit
Not the same as sample material
Extraction
What is extracted (efficiency)?
Antibody can only detect what has previously been detected