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AOAC RESEARCH INSTITUTE

AOAC

OFFICIAL METHODS OF ANALYSIS

(OMA)

OMAMAN-16: 3M Petrifilm Rapid Yeast And Mold Collaborative Study*

Study Director: Erin Crowley, Q Laboratories, 1400 Harrison Avenue, Cincinnati, OH 45214

Co-Study Director: Bob Jechorek, 3M Food Safety, 3M Center, Building 260-06-B-01, St. Paul, MN 55144

General comments about the method:

ER 1

The method is very well described in the various documents.

ER 2

NA

ER 3

The method is much simpler compared to traditional method and the space savings in the use of Petrifilm

are significant as compared to traditional agar plates.

ER 4

No additional comments

ER 5

The method is really useful due to it significantly reduces test times

ER 6

Well written and easy to follow

ER 7

none

ER 8

1. Page 7, line 17. There should be references directing the analyst to methods for the further

identification of yeast/fungal isolates. They should be ISO, BAM, MLG and others.

Pros/Strengths of the Manuscript:

ER 1

Good description of the sample preparation protocols. Efficient description of the collab study workflow

and organization. Tables are very useful for summarizing the results.

ER 2

Very well written.

ER 3

The Manuscript is well written and the information flow is in an understandable order.

ER 4

Generally well written.

ER 5

It is a simple method for working

ER 6

Very thoroughly written and detailed

ER 7

Well written in general.

ER 8

It is well written.

Cons/Weaknesses of the Manuscript:

ER 1

Very minor edits: page 7, line 25, reports lab 5 as one of the 4 labs with deviations, but in table 1 page 13,

lab 6 is marked as the lab showing deviations for ground beef??? Reading through the report and the

pack insert, it remains unclear if the minimum incubation is 48 hours or 46 hours since it is stipulated that

incubation shall be 48+/-2 hours but reading is required at 48 hours: is minimal time of 48 hours of

incubation is required?

ER 2

NA

ER 3

Table 1 describes which data sets were not used in the statistical analysis; however, there are not

indications as such in tables 2 – 9 where raw data is presented. It may help to identify the labs who’s data

sets were excluded in each table using a superscript letter.

ER 4

Need to elaborate on the issues and possible causes of those issues of laboratories whose data were not

used in the study.

ER 5

no

ER 6

None

ER 7

Page 1, line 24 states "unpaired study design" but page 4 line, line 22 states "paired study design". Please

clarify in the manuscript. Page 3, line 29: Clarify that after lyophilization dilutions were done with sterile

NFDM powder or reconstituted NFDM. Page 4, line 38: Increase font size. Page 4, line47: Justify or omit

reverse transformed mean difference here and in Tables 2014.1 and 2014.2.[Continued] Page 7, line 24:

Insert "valid" before both "data" words. Page 7, line 33: Omit Figs 1-4 which are somewhat redundant.

Add statement about acceptability of Youden plots. Page 7, line 37: Remove "reverse transformed

difference" here and in Tables 2014.1 &.2. Page 8, lines 31-33: State the repeatability SD values

supporting this assertion. Page 8, lines 44-46: State the repeatability SD values supporting this assertion.

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