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AOAC RESEARCH INSTITUTE

AOAC

OFFICIAL METHODS OF ANALYSIS

(OMA)

OMAMAN-16: 3M Petrifilm Rapid Yeast And Mold Collaborative Study*

Study Director: Erin Crowley, Q Laboratories, 1400 Harrison Avenue, Cincinnati, OH 45214

Co-Study Director: Bob Jechorek, 3M Food Safety, 3M Center, Building 260-06-B-01, St. Paul, MN 55144

ER 8

Yes

RECOMMENDATION:

Do you recommend that the ERP adopt this method as an AOAC

Official Methods of Analysis (First Action status)

?

ER 1

Yes.

ER 2

Yes

ER 3

Yes

ER 4

Yes

ER 5

yes

ER 6

Yes

ER 7

This method is potentially recommendable for adoption after manuscript clarifications are made.

ER 8

Yes, if my above comments regarding applicability and diluent are addressed. The package insert also

needs to be revised in light of these comments. If multiple diluents are recommended, even though only

1 was validated, then I would recommend against giving the method First Action Status.

AFTER FIRST ACTION STATUS:

Is there any additional information that the ERP should consider in order to recommend the method for Final Action

status?

ER 1

No.

ER 2

NA

ER 3

NA

ER 4

No additional comments

ER 5

No

ER 6

User comments

ER 7

No

ER 8

Two years worth of use in the field, without substantial problems, should be the criteria for making this a

final action method.

General Comments (3)

ER 1

ER 2

NA

ER 3

NA

ER 4

No additional comments

ER 5

ER 6

ER 7

None

ER 8

1. Package insert, page 4 of 8, Sterile Diluent. The method lists several diluents that can be used with this

method, including 0.1% peptone water. All of the diluents, other than 0.1% peptone water, should be

removed from the method, since the method was validated with 0.1 peptone water and no other diluent.

2. Package insert, page 6 of 8, applicability. The method refers to yogurt, ready-made-pie, frozen ground

beef patties, sandwiches, and dehydrated soup. These are not specific enough. Yogurt and beef should

have fat percentages. The types of pie, sandwiches and dehydrated soup should be specified. For

example, apple pie, chicken soup, tuna sandwich... The applicability is too broad as written.

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