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3M Petrifilm RYM Collaborative Study June 2014
OMA-2014-June-XXX
difference in performance for three test parameters: incubation time, incubation
temperature and type of diluents used to rehydrate the plate medium.
The method was awarded PTM certification number 121301 on December 18,
2013.
1.3 Study Directors for Collaborative Study
Robert P. Jechorek Patrick Bird & Erin Crowley
Study Co-Director Study Co-Directors
3M Food Safety Department Q Laboratories, Inc.
3M Center, Building 260-6B-01 1400 Harrison Avenue
St. Paul, MN 55144 Cincinnati, Ohio 45214
Phone: 651-733-9764 Phone : 513-471-1300
Fax: 651-733-5819 Fax : 513-471-5600
Email: Email:
2. Collaborators
A total of 12-13 collaborators will be solicited to participate in this study. A minimum of
8 laboratories, with acceptable data, will be needed for the successful completion of this
study. Qualified laboratories currently using the 3M Petrifilm Plates or who will be
thoroughly trained by a 3M representative, will act as collaborators for this study.
A sample letter to collaborators can be found in Appendix 8.1. This letter will be sent to
each collaborator prior to the start of the study.
3. Collaborative Study Design
1.1. This collaborative study is designed according to the AOAC INTERNATIONAL
Methods Committee Guidelines for Validation of Microbiological Methods for
Food and Environmental Surfaces (2012).
Table 1. Overview of Study Design
MATRIX Inoculating
Organism
Target
Levels
# Replicates
SAMPLE
CODES
3M
RYM*
ISO/
BAM
*
Raw frozen
ground
Trichosporon
mucoides
0 cfu/g 4 3 Random
number
~50 cfu/g 4 3
5
DRAFT DOCUMENT
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16A/ Collaborative Study Protocol
ERP Use Only - December 2014