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3M Petrifilm RYM Collaborative Study
June 2014
OMA-2014-June-XXX
ATCC 201382), manually mixed and held at -20 to -30°C for a
minimum of 2 weeks.
4.1.3.2.
Raw almonds
will be inoculated with a lyophilized culture
(
Aspergillus aculeatus
ATCC 56925) and stabilized for a minimum
of 2 weeks at room temperature (21-23°C).
4.2.
Shipment of Test portions
4.2.1. A total of 16 test portions (2 test portions x 4 levels x 2 methods) of each
matrix will be sent to each laboratory.
4.2.2. Test portions will be shipped in leak-proof, insulated containers with ice
packs to maintain the appropriate temperature range.
4.2.3. Packages will be sent via overnight courier.
4.3.
Test Portion Analysis
4.3.1. The laboratories will follow the appropriate test portion preparation
protocol according to the method for each food.
5.
3M Petrifilm RYM Count Plate
5.1.
Applicability
For the enumeration of yeast and mold from yogurt, sour cream, raw almonds,
sliced apples, frozen bread dough, ready-made pie, sandwiches, dehydrated soup,
fermented salami and frozen ground beef patties (80% lean/20% fat).
Caution
:
3M Petrifilm RYM Plates do not differentiate any one yeast or mold strain from
another. 3M has not documented 3M Petrifilm RYM Plates for use in industries
other than food and beverage. For example, 3M has not documented 3M Petrifilm
RYM Plates for testing water, pharmaceuticals or cosmetics. 3M Petrifilm RYM
Plates have not been tested with all possible food products, food processes, testing
protocols or with all possible strains of yeast and mold. Do not use 3M Petrifilm
RYM Plates in the diagnosis of conditions in humans or animals. The user must
train its personnel in proper testing techniques. After use, 3M Petrifilm RYM
Plates may contain microorganisms that may be a potential biohazard. Follow
current industry standards for disposal.
For information on potential biohazards,
reference Biosafety in Microbiological and Biomedial Laboratories, 5th edition,
Section VIII-B: Fungal Agents.
5.2.
Test Kit -
3M™
Petrifilm™ Rapid Yeast and Mold Count Plate
7
DRAFT DOCUMENT
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16A/ Collaborative Study Protocol
ERP Use Only - December 2014