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AOAC RESEARCH INSTITUTE

AOAC

OFFICIAL METHODS OF ANALYSIS

(OMA)

OMAMAN-16: 3M Petrifilm Rapid Yeast And Mold Collaborative Study*

Study Director: Erin Crowley, Q Laboratories, 1400 Harrison Avenue, Cincinnati, OH 45214

Co-Study Director: Bob Jechorek, 3M Food Safety, 3M Center, Building 260-06-B-01, St. Paul, MN 55144

ER 7

In Table 2014.1 for the RYM method it is not clear what data from Tables 2, 3, 4 & 5 is used to get the SD

values for repeatability and reproducibility. In Table 2014.2 for the RYM method it is not clear what data

from Tables 6 7, 8 & 9 is used to get the SD values presented for repeatability and reproducibility.

Typically the SD for reproducibility is noticeably larger than that for repeatability. This is true for the

2014.2 (almond) results by the RYM and the FDA/ISO methods. However, it is not so for the 2014.1 (beef)

results by the RYM or reference method. So perhaps the calculations and data inputs should be checked.

[Continued] In many cases, especially in Table 2014.1 the mean RYM and reference methods' mean

counts are not significantly different and yet the differences, although not very different, are often

significantly different. In Table 2014.1 perhaps this is related to the fact that only 11 collaborators

provided valid results.

ER 8

None.

General Comments (2)

ER 1

ER 2

NA

ER 3

NA

ER 4

No additional comments

ER 5

ER 6

ER 7

None

ER 8

Tables 2014.1 & 2014.2. It should be footnoted that the BAM and ISO methods are identical when using

0.1 % peptone water. as was done in this collaborative study.

EDITORIAL EVALUATION CRITERIA

Is the Validation Study Manuscript in a format acceptable to AOAC?

ER 1

Yes

ER 2

Yes

ER 3

Yes

ER 4

Yes

ER 5

Yes

ER 6

Yes

ER 7

Yes

ER 8

Yes

Is the method described in sufficient detail so that it is relatively easy to understand, including equations and procedures

for calculation of results (are all terms explained)?

ER 1

Yes

ER 2

Yes

ER 3

Yes

ER 4

Yes

ER 5

Yes

ER 6

Yes

ER 7

No

ER 8

Yes

Are the figures and tables sufficiently explanatory without the need to refer to the text?

ER 1

Yes

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