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3M Petrifilm RYM Collaborative Study
June 2014
OMA-2014-June-XXX
Appendix 8.3
Study Materials
Materials and reagents provided by the study sponsor
1.
3M™
Petrifilm™
Flat Spreader (6425)
2.
3M™
Petrifilm™ Rapid Yeast and Mold Count Plates
3.
Sterile diluent –
0.1% peptone water
4.
Dichloran-rose bengal chloramphenicol agar (DRBC)
5.
Dichloran 18% glycerol (DG18) agar
6.
L shaped spreaders (hockey sticks)
Materials required but not provided
1.
Pipettes
. –
capable of pipetting 1,000µL or sterile serological pipette
2.
Sterile pipette tips.
–
capable of 1,000µL
3.
Stomacher®
. –
Seward or equivalent
4.
Filter Stomacher®
bags
. - Seward or equivalent
5.
Incubators
. –
Incubator capable of maintaining 25 ±
1°C and another capable of
maintaining 28 ±
1°C, and having a solid front so as to maintain a dark interior.
6.
Refrigerator
. –
capable of maintaining 2-8°C, for storing the 3M Petrifilm RYM
Count Plates
18
DRAFT DOCUMENT
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16A/ Collaborative Study Protocol
ERP Use Only - December 2014