Method Submissions-5th Set of Nutrients_6-30-14 - page 54

6
(mg/L);
V
——fixed volume of the test sample, inmilliliters (mL);
10——fixed volume of the enzymatic hydrolyzate of the test sample, inmilliliters (mL);
k
1
——correct thewatermolecules increased during the hydrolysis of fructan;
k
2
——correct type F
n
terminal fructose reducing in fructan and undetection of typeGF
n
terminal glucose in fructan;
n
——average polymerization degree. For inulin,
n
=10. For fructooligofructose,
n
=4;
m
——test sampleweight, in grams (g);
0.2——the volume of test sample solution for enzyme hydrolysis, inmilliliters (mL);
10000——unity conversion coefficient.
The computed result is presented as the arithmeticalmean of the results of two independent
determinations acquired under repeat conditions, retain three significant figures.
7 Precisiondegree
Absolute difference of the results of two independent determinations acquired under repeat
conditions should not exceed 10%of the arithmeticmean.
8 Others
The limit of detection in this standard is 0.04 g/100g*, and the limit of quantitation is 0.2
g/100g*.
*concentrations apply to solid powders, if apply to reconstituted powders(25 g into 200gof
water), the limit of detection is 0.005 g/100g and the limit of quantitation is 0.022
g/100g.
FOS-02
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